Live Imaging

Two-photon microscope

The microscope is an inverted Axio-Observer Z1 from Zeiss, equipped with a Chameleon system Ultra (I) Titane Sapphire 80 Mhz laser. Tuning range wavelength : 690 to 1040 nm.

• One monophoton laser at 543 nm
• Software is Zeiss LSM 510.
• Microscope with motorized objective nosepiece.
• Plan Apo-Chromat 10x/0.45, without immersion
• Plan Apo-Chromat 20x/0.8, without immersion
• LD-C-Apochromat 40x/1.1 w korr UV-Vis_IR, water immersion motorized stage.

Controlled environment:

• The system is equipped with a large volume incubator from Okolab maintaining temperature at 37°C.
• Custom design gas micro-chamber is installed. The Gas micro-chamber fits on the microscope stage, inside the temperature controlled enclosure, and hosts the sample. It creates a small volume inside the enclosure in which the pre-mixed and pre-humidified stream of gas is continuously fed.
• The gas chamber can receive, either two multi well plates or eight 35mm Petri dish or six chambers slides system.
• The environmental air is controlled with a 3 gas mixer. The mixed gas flows through a humidifier module.

Time lapse videomicroscopes

1 / Nikon

• The microscope is an inverted Eclipse Ti from Nikon.
• Imaging software: NIS Elements
• Camera: ANDOR Technology, Clara Series.
• Microscope with motorized objective nosepiece.
• PLAN Fluor 4x/0.1 PhL DL
• PLAN Fluor 10x/0.3 Ph1 DL
• S PLAN Fluor ELWD 20x/0.5 DIC N1
• S PLAN Fluor ELWD 20x/0.5 Ph1 ADM
• S PLAN Fluor ELWD 40x/0.6 DIC N1
• S PLAN Fluor ELWD 40x/0.6 Ph2 ADM
• All objectives are used without immersion.
• Motorized stage.
• Several fluorescences :

Six filters were installed on the machine and it is possible to install two more filters.

Controlled environment:

• The system is equipped with a large volume incubator from LIS (Life Imaging Services) maintaining temperature at 37°C.
• A gas micro-chamber is installed inside the temperature controlled incubator.
• The gas micro-chamber can receive multiple stage inserts:
  • PetriDish,
  • Multi well plate
  • Chamber Slides system
  • IVF 4 well Dishes
• The environmental air is controlled with a constant CO2 percentage. The mixed gas flows through a humidifier module.

2 / LEICA

• The microscope is an inverted DMIRBE from LEICA.
• Imaging software : Metamorph with the ND acquisition module
• Camera: Photometrics CoolSnap ES.
• Microscope with motorized objective nosepiece.
• C PLAN 10x/0.22 LMC
• C PLAN 20x/0.3 LMC
• N-Plan 10x/0.25 PH1
• N-Plan 20x/0.40 corr PH1
• N-Plan 40x/0.55 corr PH2
• All objectives are used without immersion.
• Motorized stage.

Controlled environment:

• The system is equipped with a small incubator from PECON maintaining temperature at 37°C and constant CO2 percentage.
• 2 Heating inserts: 6 Multi well plates or Petri dish (60mm)
• Temperature and gas controller come from Pecon.