Building the cerebral cortex: Cell and molecular mechanisms
Our team has made significant contributions on the role of cell cycle control in corticogenesis and the discovery of unique features of early primate development. We are one of the few groups that bridges the gap between the extensive work world-wide on the rodent-model of corticogenesis and the few labs able to work on human corticogenesis. Our work shows that the non human primate corticogenesis differs markedly form that in rodent and shares key features with the human primate.

We have established major differences in the nature and timing of the ontogenetic processes that characterize primate corticogenesis and were the first to identify a human and non-human primate-specific germinal zone: the Outer Sub Ventricular Zone (OSVZ) (Smart et al., 2002) . We have shown that the primate OSVZ includes a high diversity of progenitors including five morphotypes, each characterized by distinct proliferative behaviors. State transition analysis of a large database of lineage trees unexpectedly revealed frequent bidirectional transitions between progenitor types, not observed in other mammalian species (Betizeau et al., Neuron, 2013; Pfeiffer et al., J Comp Neurol, 2016).

We have discovered that primate-specific miRNA signatures uniquely distinguish VZ and OSVZ. Many of these primate-specific miRNAs target cell-cycle genes, indicating that each germinal zone has evolved its own cell-cycle regulation scheme. This suggests the evolution of a complex regulatory control system that may have contributed to the emergence of novel progenitor types interacting through complex lineages. (Arcila et al. Neuron, 2014; Dehay et al., Neuron, 2015)

One of  major contributions include discovery of the role of cell-cycle regulation in establishing cortical architecture and cell lineage evolution, via control of cortical progenitor pool amplification and on mode of division (Pilaz et al., PNAS, 2009; Lukaszewicz et al., Neuron, 2005; Dehay and Kennedy, Nat Rev Neurosci, 2007). We have shown that a conserved mechanism, the asymmetrical spindle morphology (SSA) , known to be a core component of ACD in invertebrates is implicated in mammalian corticogenesis where it regulates cell-cycle exit and asymmetric division in apical progenitors of the VZ (Delaunay et al., 2014, Cell Reports, 2014; Delaunay et al., Curr Opin Neurobiol, 2017).

During the last decade, we have established the first line of rhesus ES cells, which we are currently using to generate 3D cortical organoids (Wianny et al., Stem Cells 2008; Stem Cell Res, 2016)

2024Colette Dehay, Wieland B HuttnerDevelopment and evolution of the primate neocortex from a progenitor cell perspectiveDevelopment
2023Alexandra A de Sousa, Amélie Beaudet, Tanya Calvey, Ameline Bardo, Julien Benoit, Christine J Charvet, Colette Dehay, Aida Gómez-Robles, Philipp Gunz, Katja Heuer, Martijn P van den Heuvel, Shawn Hurst, Pascaline Lauters, Denné Reed, Mathilde Salagnon, Chet C Sherwood, Felix Ströckens, Mirriam Tawane, Orlin S Todorov, Roberto Toro, Yongbin WeiFrom fossils to mindCommun Biol
2022Wianny F, Dzahini K, Fifel K, Wilson CRE, Bernat A, Dolmazon V, Misery P, Lamy C, Giroud P, Cooper HM, Knoblauch K, Procyk E, Kennedy H, Savatier P, Dehay C, Vezoli JInduced Cognitive Impairments Reversed by Grafts of Neural Precursors: A Longitudinal Study in a Macaque Model of Parkinson's DiseaseAdv Sci (Weinh)
2021Irène Aksoy, Cloé Rognard, Anaïs Moulin, Guillaume Marcy, Etienne Masfaraud, Florence Wianny, Véronique Cortay, Angèle Bellemin-Ménard, Nathalie Doerflinger, Manon Dirheimer, Chloé Mayère , Pierre-Yves Bourillot, Cian Lynch, Olivier Raineteau, Thierry Joly, Colette Dehay, Manuel Serrano, Marielle Afanassieff, Pierre SavatierApoptosis, G1 Phase Stall, and Premature Differentiation Account for Low Chimeric Competence of Human and Rhesus Monkey Naive Pluripotent Stem CellsStem Cell Reports
2021Sisu Han, Satoshi Okawa, Grey Atteridge Wilkinson, Hussein Ghazale, Lata Adnani, Rajiv Dixit, Ligia Tavares, Imrul Faisal, Matthew J Brooks, Veronique Cortay, Dawn Zinyk, Jinghua Gao, Vorapin Chinchalongporn, Ana-Maria Oproescu, Lakshmy Vasan , Yacine Touahri, Luke Ajay David, Eko Raharjo, Jung-Woong Kim, Wei Wu, Waleed Rahmani, Jennifer Ai-Wen Chan, Igor Kovalchuk, Liliana Attisano, Deborah Kurrasch, Colette Dehay, Anand Swaroop, Diogo S Castro, Jeff Biernaskie , Antonio Del Sol, Carol SchuurmansProneural genes define ground-state rules to regulate neurogenic patterning and cortical foldingNeuron
2020Magrou L, Barone P, Markov NT, Scheeren G, Killackey HP, Giroud P, Berland M, Knoblauch K, Dehay C*, Kennedy H*, *co-senior authorsUnique Features of Subcortical Circuits in a Macaque Model of Congenital BlindnessCereb Cortex
2020Luanda Lins, Florence Wianny, Colette Dehay, Jacques Jestin, Watson LohAdhesive Sponge Based on Supramolecular Dimers Interactions as Scaffolds for Neural Stem CellsBiomacromolecules
2020Ribeiro Gomes AR, Olivier E, Killackey HP, Giroud P, Berland M, Knoblauch K, Dehay C*, Kennedy H*, *co-senior authors, F1000 recommandationRefinement of the Primate Corticospinal Pathway During Prenatal Development.Cereb Cortex
2019Arai Y, Cwetsch AW, Coppola E, Cipriani S, Nishihara H, Kanki H, Saillour Y, Freret-Hodara B, Dutriaux A, Okada N, Okano H, Dehay C, Nardelli J, Gressens P, Shimogori T, D'Onofrio G, Pierani AEvolutionary Gain of Dbx1 Expression Drives Subplate Identity in the Cerebral Cortex.Cell Rep
2019Fousse J, Gautier E, Patti D, Dehay CDevelopmental changes in interkinetic nuclear migration dynamics with respect to cell-cycle progression in the mouse cerebral cortex ventricular zone.J Comp Neurol