Molecular Biology Facility

NUCLEIC ACID ANALYSIS

• Nucleic Acid Quantitation and Quality Control
  • BioPhotometer (Eppendorf)
  • NanoDrop Spectrophotometer (Life’s)
  • Qubit 2.0 Fluorometer (Invitrogen)
• PCR Machines
  • T3 Thermocycler (Biometra)
  • T3000 Thermocycler (Biometra)
  • PTC-200 (MJ Research)
  • Gene Touch (Bio Concept)
• Real Time PCR Machines
  • LightCycler 1.5 (Roche)
  • StepOnePlus (Applied BioSystems) (x2)
  • Mx3000P (Stratagene)
• PCR Workstation
  • Workstation 36”wide (CBS Scientific)
  • Standard UV PCR Systems (UVP)
• Gel Electrophoresis Systems
• Digital Imaging System
  • Mega Bio-Print (Biotech)

PROTEIN ANALYSIS

• PAGE and Western Blotting Apparatus
  • Mini Protean System (Bio-Rad)
  • Protean II xi Cell (Bio-Rad)
  • Trans-Blot Turbo Transfer System (Bio-Rad)
  • SNAP i.d. Protein Detection System (Millipore)
• Digital Imaging System
  • ChemiDoc MP Imaging system (Bio-Rad)

PROTEIN PURIFICATION

• HPLC
  • Ultimate 3000 Pump (Dionex)
  • Spectra System AS3500 Injector (Thermo Separation Products)
  • Coulochem III Electrochemical Detector (Dionex)

MICROPLATE READERS

• Luminometer
  • Glomax Multi Detection System (Promega)

BACTERIA CULTURE (L1 SAFETY LAB)

• Incubator
  • Innova 4080 Shaker (New Brunswick)
  • C25 Incubator Shaker (New Brunswick)
  • Heratherm Advanced Protocol Microbiological Incubator (ThermoScientific)
• Class II Biosafety Cabinet
  • MSC Advantage ((ThermoScientific)
• Centrifuge
  • Avanti J-E High Speed (Beckman Coulter)

CELL ANALYSER

• LSR Fortessa X20 4 lasers (BD Biosciences)

ULTRASONICATOR

• Bioruptor Plus (Diagenode)

LASER CAPTURE MICRODISSECTION

The microscope is an upright Leica DM6000 B.

Microscope with motorized objective nosepiece:

• One objective for the overview:
  • 1.25x/0.04 HCX PL FLUOTAR
• Five objectives for microdissection:
  • 5x/0.12 UVI Microdissection
  • 10x/0.30 HC PL FLUOTAR
  • 20x/0.40 HCX PL FL L CORR PH1
  • 40x/0.60 HCX PL FL L CORR PH2
  • 63x/0.70 HCX PL FL L CORR XT
• Camera Leica DFC 310FX
• Camera LMD CC7000 digital color
• Fluorescence source EL6000 with three filters:
  • LMD-DAPI
  • LMD-GFP
  • LMD-RFP
• UV laser for microdissection
• Motorized stage with a three slides holder
• Two collectors for tube caps:
  • 4x 0.2ml PCR tubes
  • 4x 0.5ml PCR tubes
• Leica LMD Software

GENERAL EQUIPEMENT

• Hybridisation Ovens
  • UNB100 (Memmert)
  • UNB200 (Memmert)
  • UFE400 (Memmert)
  • Heratherm (Thermoscientific)
  • HybEZ Oven (ACD Advanced Cell Diagnostics)
• -80°C Freezer
  • HFU T Series (Thermoscientific) (x2)
  • HERA FREEZE BASIC (Thermoscientific)
  • VIP SERIES (Sanyo) (x2)
  • HEF U570 (New Brunswick)
  • Innova U535 (New Brunswick)
  • -80°C FREEZER (FormaScientific)
  • HERAfreeze (Heraeus)

DATA ANALYSIS SOFTWARE

• FlowJo (FlowJo)
• BD FACSDIVATM SOFTWARE (BD Biosciences)