Pluripotent stem cells in mammals

We explore the mechanisms of self-renewal and pluripotency using embryonic stem cells from several mammalian sepcies. In particular, we explore ground state pluripotency in the rabbit and primate pre-implantation embryos.

Our goal is to create rabbit and macaque ES cells capable of colonizing the developing embryo, suitable for the generation of chimaeras.

Another aspect of our work focuses on the regulation of the cell cycle, which shows unusual characteristics in pluripotent stem cells.

2019Gonnot F, Langer D, Bourillot PY, Doerflinger N, Savatier PRegulation of Cyclin E by transcription factors of the naïve pluripotency network in mouse embryonic stem cellsCell Cycle
2018Afanassieff M, Aksoy I, Beaujean N, Bourillot PY, Savatier PFifty shades of pluripotencyMed Sci (Paris)
2017Savatier P, Osteil P, Tam PPPluripotency of embryo-derived stem cells from rodents, lagomorphs, and primates: Slippery slope, terrace and cliffStem Cell Res
2017Tapponnier Y, Afanassieff M, Aksoy I, Aubry M, Moulin A, Medjani L, Bouchereau W, Osteil P, Nurse-Francis J, Oikonomakos I, Joly T, Jouneau L, Archilla C, Schmaltz-Panneau B, Peynot N, Barasc H, Pinton A, Lecardonnel J, Gocza E, Beaujean N, Duranthon V, Savatier PReprogramming of rabbit induced pluripotent stem cells toward epiblast and chimeric competency using Krüppel-like factorsStem Cell Res
2016Osteil P, Moulin A, Santamaria C, Joly T, Jouneau L, Aubry M, Tapponnier Y, Archilla C, Schmaltz-Panneau B, Lecardonnel J, Barasc H, Mouney Bonnet N, Genthon C, Roulet A, Donnadieu C, Acloque H, Gocza E, Duranthon V, Afanassieff M, Savatier PA Panel of Embryonic Stem Cell Lines Reveals the Variety and Dynamic of Pluripotent States in RabbitsStem Cell Reports
2015Chen H, Aksoy I, Gonnot F, Osteil P, Aubry M, Hamela C, Rognard C, Hochard A, Voisin S, Fontaine E, Mure M, Afanassieff M, Cleroux E, Guibert S, Chen J, Vallot C, Acloque H, Genthon C, Donnadieu C, De Vos J, Sanlaville D, Guérin JF, Weber M, Stanton LW, Rougeulle C, Pain B, Bourillot PY, Savatier PReinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotencyNat Commun
2014Aksoy I, Giudice V, Delahaye E, Wianny F, Aubry M, Mure M, Chen J, Jauch R, Bogu GK, Nolden T, Himmelbauer H, Xavier Doss M, Sachinidis A, Schulz H, Hummel O, Martinelli P, Hübner N, Stanton LW, Real FX, Bourillot PY, Savatier PKlf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in embryonic stem cellsNat Commun
2013Coronado D, Godet M, Bourillot PY, Tapponnier Y, Bernat A, Petit M, Afanassieff M, Markossian S, Malashicheva A, Iacone R, Anastassiadis K, Savatier PA short G1 phase is an intrinsic determinant of naïve embryonic stem cell pluripotencyStem Cell Res
2013Osteil P, Tapponnier Y, Markossian S, Godet M, Schmaltz-Panneau B, Jouneau L, Cabau C, Joly T, Blachère T, Gócza E, Bernat A, Yerle M, Acloque H, Hidot S, Bosze Z, Duranthon V, Savatier P, Afanassieff MInduced pluripotent stem cells derived from rabbits exhibit some characteristics of naïve pluripotencyBiol Open
2010Niu Y, Yu Y, Bernat A, Yang S, He X, Guo X, Chen D, Chen Y, Ji S, Si W, Lv Y, Tan T, Wei Q, Wang H, Shi L, Guan J, Zhu X, Afanassieff M, Savatier P, Zhang K, Zhou Q, Ji WTransgenic rhesus monkeys produced by gene transfer into early-cleavage-stage embryos using a simian immunodeficiency virus-based vectorProc Natl Acad Sci U S A