Cellular programming in the brain

Our laboratory investigates lineage specification in the postnatal SVZ, the main germinal zone of the postnatal forebrain. We have participated to the demonstration that the SVZ is not a homogeneous germinal region, but is composed of microdomains that have different embryonic origins, distinct transcriptional signatures and are biased in generating specific neural cell types (reviewed in (Fiorelli et al 2015)).

Over the last few years, we have progressively focused on characterizing the dorsal-most domain of the postnatal SVZ (dorsal SVZ or dVSZ). This region is particularly interesting as it continues producing cells of distinct lineages, including distinct neuronal lineages. In particular, this region contains progenitors of oligodendrocytes and glutamatergic neurons, two cells type that are particularly sensitive to degeneration in a variety of pathologies.

By using a combination of molecular and imaging tools, our research addresses the following aims:

  • To better understand neural lineages diversity as well as their transcriptional coding in the postnatal forebrain
  • To manipulate postnatal progenitors fate in health but also in pathologies such as neonatal hypoxia, in order to promote CNS repair
  • To study the potency of postnatal progenitors to produce subtypes of functional neurons an glial cells
2019Marcy G, Raineteau OContributions of Single-Cell Approaches for Probing Heterogeneity and Dynamics of Neural Progenitors Throughout Life: Concise Review.Stem Cells
2018Zweifel S, Buquet J, Caruso L, Rousseau D, Raineteau O"FlashMap" - A Semi-Automatic Tool for Rapid and Accurate Spatial Analysis of Marker Expression in the Subventricular ZoneSci Rep
2018Benito N, Gaborieau E, Sanz Diez A, Kosar S, Foucault L, Raineteau O, De Saint Jan DA Pool of Postnatally Generated Interneurons Persists in an Immature Stage in the Olfactory BulbJ Neurosci
2018Gaborieau E, Hurtado-Chong A, Fernández M, Azim K, Raineteau OA dual role for the transcription factor Sp8 in postnatal neurogenesisSci Rep
2018Zweifel S, Marcy G, Lo Guidice Q, Li D, Heinrich C, Azim K, Raineteau OHOPX Defines Heterogeneity of Postnatal Subventricular Zone Neural Stem CellsStem Cell Reports
2018Donega V, Marcy G, Lo Giudice Q, Zweifel S, Angonin D, Fiorelli R, Abrous DN, Rival-Gervier S, Koehl M, Jabaudon D, Raineteau OTranscriptional Dysregulation in Postnatal Glutamatergic Progenitors Contributes to Closure of the Cortical Neurogenic PeriodCell Rep
2017Azim K, Angonin D, Marcy G, Pieropan F, Rivera A, Donega V, Cantu C, Williams G, Berninger B, Butt AM, Raineteau OPharmacogenomic identification of small molecules for lineage specific manipulation of subventricular zone germinal activityPLoS Biol
2017Donega V, Raineteau OPostnatal Neural Stem Cells: Probing their competence for cortical repairThe Neuroscientist
2015Azim K, Hurtado-Chong A, Fischer B, Kumar N, Zweifel S, Taylor V, Raineteau OTranscriptional Hallmarks of Heterogeneous Neural Stem Cell Niches of the Subventricular ZoneStem Cells
2015Fiorelli R, Fischer B, Azim K, Raineteau OAdding a spatial dimension to postnatal ventricular-subventricular zone neurogenesisDevelopment